30^th Experts Meet On Cancer Therapy

Biography

Biography: Yeni Purnamasari

Abstract

Coelomic fluid of the earthworm had a lot of bioactive substances as therapeutic drug and anticancer effect that has been proven in many medical research.  The benefit of coelomic fluid as anticancer effect needs further investigation. This study aimed to investigate whether the coelomic fluid of the earthworm Lumbricus rubellus as combination of 5-Fluouracil has role in decreasing focal adhesion kinase (FAK) and calcium intracellular expression (iCa^2-) on colorectal cancer HT-29 cell line. HT-29 cells were cultured by adding coelomic fluid with 5-Fluouracil (5,10, and 20 g/ml) and without 5-Fluouracil (0, 20 g/ml) for 24 hours. We assessed the percentage of FAK level using flowcytometri and iCa²⁺ using immune-fluorescence. The result showed that after treating HT-29 cells with combination of coelomic fluid and 5-Fluouracil in different concentration, the percentage FAK level significantly decreased in dose combination (p < 0.05) and iCa²⁺ percentage compare each groups was significantly differences (p< 0.05). Corelation between percentage FAK and iCa²⁺ showed that there is a strong correlation (R = 0.77). This suggest that treating combination coelomic fluid and 5-fluouracil in HT-29 cells made the decrease in FAK percentage and also followed by iCa²⁺ percentage. Our finding suggested that coelomic fluid of the earthworm Lumbricus rubellus could prove useful as a combination of 5-fluouracil in colorectal cancer therapy.